Structure and effective charge characterization of proteins by a mobility capillary electrophoresis based method
نویسندگان
چکیده
منابع مشابه
A stability indicating capillary electrophoresis method for analysis of buserelin
A simple and rapid stability indicating method based on capillary zone electrophoresis has been developed and validated for the analysis of buserelin (BUS). The best separations were achieved by using a bare fused silica capillary (75 m i.d.; 65.5 cm total, 57.0 cm effective length), phosphate buffer (pH = 3.00; 26.4 mM), at 35 °C. The sample was hydrodynamically injected at 50 mbar for 5 seco...
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Almost all proteins contain charged amino acids. While the function in catalysis or binding of individual charges in the active site can often be identified, it is less clear how to assign function to charges beyond this region. Are they necessary for solubility? For reasons other than solubility? Can manipulating these charges change the properties of proteins? A combination of capillary elect...
متن کاملA stability indicating capillary electrophoresis method for analysis of buserelin
A simple and rapid stability indicating method based on capillary zone electrophoresis has been developed and validated for the analysis of buserelin (BUS). The best separations were achieved by using a bare fused silica capillary (75 m i.d.; 65.5 cm total, 57.0 cm effective length), phosphate buffer (pH = 3.00; 26.4 mM), at 35 °C. The sample was hydrodynamically injected at 50 mbar for 5 seco...
متن کاملDetermination of the effective charge of a protein in solution by capillary electrophoresis.
This paper describes two methods to estimate the effective charge of a protein in solution by capillary electrophoresis and demonstrates these methods by using representative proteins. In one method, a "charge ladder"--a series of derivatives of a protein differing by known increments of charge but differing only minimally in hydrodynamic drag--is generated by covalent modification of the epsil...
متن کاملCapillary Electrophoresis of PEGylated Proteins
Capillary electrophoresis has become a well-established technique for evaluating the composition and concentrations of a wide range of protein samples. In addition, capillary electrophoresis can be used to study a variety of protein properties including the net protein charge, the extent of ion binding, and the presence of specific biomolecular interactions. This wide range of applications is p...
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ژورنال
عنوان ژورنال: Chemical Science
سال: 2019
ISSN: 2041-6520,2041-6539
DOI: 10.1039/c9sc02039j